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p egfr  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc p egfr
    MFAP2 promotes epithelial–mesenchymal transition (EMT) through the <t>EGFR-AKT-STAT3</t> signaling pathway in colorectal cancer (CRC) cells. (A, B) The top 20 enrichment signaling pathways regulated by MFAP2 knockdown. (C, D) VEGFR2 signaling pathway was enriched in the MFAP2 knockdown cells, shown by Gene Set Enrichment Analysis (GSEA) and essential genes in this enrichment. (E) MFAP2 knockdown affected the EGFR-AKT-STAT3 axis.
    P Egfr, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 4192 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/egfr+p/EGF+Receptor+Antibody/pmc12914112-62-65-66
    Average 97 stars, based on 4192 article reviews
    p egfr - by Bioz Stars, 2026-09
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    Images

    1) Product Images from "MFAP2 promotes metastasis and drug resistance by regulating epithelial-to-mesenchymal transition through EGFR signaling pathway in colorectal cancer cells"

    Article Title: MFAP2 promotes metastasis and drug resistance by regulating epithelial-to-mesenchymal transition through EGFR signaling pathway in colorectal cancer cells

    Journal: Genes & Diseases

    doi: 10.1016/j.gendis.2025.101800

    MFAP2 promotes epithelial–mesenchymal transition (EMT) through the EGFR-AKT-STAT3 signaling pathway in colorectal cancer (CRC) cells. (A, B) The top 20 enrichment signaling pathways regulated by MFAP2 knockdown. (C, D) VEGFR2 signaling pathway was enriched in the MFAP2 knockdown cells, shown by Gene Set Enrichment Analysis (GSEA) and essential genes in this enrichment. (E) MFAP2 knockdown affected the EGFR-AKT-STAT3 axis.
    Figure Legend Snippet: MFAP2 promotes epithelial–mesenchymal transition (EMT) through the EGFR-AKT-STAT3 signaling pathway in colorectal cancer (CRC) cells. (A, B) The top 20 enrichment signaling pathways regulated by MFAP2 knockdown. (C, D) VEGFR2 signaling pathway was enriched in the MFAP2 knockdown cells, shown by Gene Set Enrichment Analysis (GSEA) and essential genes in this enrichment. (E) MFAP2 knockdown affected the EGFR-AKT-STAT3 axis.

    Techniques Used: Protein-Protein interactions, Knockdown

    Related Articles

    Immunofluorescence:

    Article Title: Protein-Bound Uremic Toxins Induce Tissue Remodeling by Targeting the EGF Receptor
    Article Snippet: The Ventana BenchMark automated staining system and Ventana reagents were used (Ventana Medical Systems, Tucson, AZ), and primary antibodies against MMP2/MMP9 (1:100 dilution; Abcam, Inc.) and β -catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology) at 4°C were used overnight. .. For immunofluorescence staining, cultured HK2 cells and serial cryostat sections of renal cortex were incubated with a primary rabbit antibody against EGFR (1:100 dilution; Cell Signaling Technology), EGFR-p (Tyr1068, 1:100 dilution; Cell Signaling Technology), and β -catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology). .. For renal tubule segment identification, frozen kidney samples were incubated with primary antibodies including FITC-conjugated anti- Lotus tetragonolobus lectin (1:500 dilution; Vector Laboratories, Burlingame, CA) and FITC-conjugated anti- Dolichosbiflorus agglutinin (1:500 dilution; Vector Laboratories).

    Article Title: Protein-Bound Uremic Toxins Induce Tissue Remodeling by Targeting the EGF Receptor
    Article Snippet: For immunohistochemistry analysis, paraffin tissue sections were cut, mounted, deparaffi- nized, rehydrated, and stained with hematoxylin and eosin by using standard histologic techniques.TheVentanaBenchMark automated staining system and Ventana reagents were used (VentanaMedical Systems, Tucson, AZ), and primary antibodies against MMP2/ MMP9 (1:100 dilution; Abcam, Inc.) and b-catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology) at 4°C were used overnight. .. For immunofluorescence staining, cultured HK2 cells and serial cryo- stat sections of renal cortex were incubated with a primary rabbit antibody against EGFR (1:100 dilution; Cell Signaling Technology), EGFR-p (Tyr1068, 1:100 dilution; Cell Signal- ing Technology), and b-catenin-p (Ser33/ Ser37, 1:100 dilution; Cell Signaling Technology). .. For renal tubule segment identification, frozen kidney sampleswere incubatedwith pri- mary antibodies including FITC-conjugated anti-Lotus tetragonolobus lectin (1:500 dilu- tion; Vector Laboratories, Burlingame, CA) and FITC-conjugated anti-Dolichosbiflorus agglutinin (1:500 dilution; Vector Laboratories).31 The sections were counterstained with 49,6-diamidino-2-phenylindole (dilu- tion 1:500; Sigma-Aldrich) to identify cellular nuclei.

    Staining:

    Article Title: Protein-Bound Uremic Toxins Induce Tissue Remodeling by Targeting the EGF Receptor
    Article Snippet: The Ventana BenchMark automated staining system and Ventana reagents were used (Ventana Medical Systems, Tucson, AZ), and primary antibodies against MMP2/MMP9 (1:100 dilution; Abcam, Inc.) and β -catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology) at 4°C were used overnight. .. For immunofluorescence staining, cultured HK2 cells and serial cryostat sections of renal cortex were incubated with a primary rabbit antibody against EGFR (1:100 dilution; Cell Signaling Technology), EGFR-p (Tyr1068, 1:100 dilution; Cell Signaling Technology), and β -catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology). .. For renal tubule segment identification, frozen kidney samples were incubated with primary antibodies including FITC-conjugated anti- Lotus tetragonolobus lectin (1:500 dilution; Vector Laboratories, Burlingame, CA) and FITC-conjugated anti- Dolichosbiflorus agglutinin (1:500 dilution; Vector Laboratories).

    Article Title: Protein-Bound Uremic Toxins Induce Tissue Remodeling by Targeting the EGF Receptor
    Article Snippet: For immunohistochemistry analysis, paraffin tissue sections were cut, mounted, deparaffi- nized, rehydrated, and stained with hematoxylin and eosin by using standard histologic techniques.TheVentanaBenchMark automated staining system and Ventana reagents were used (VentanaMedical Systems, Tucson, AZ), and primary antibodies against MMP2/ MMP9 (1:100 dilution; Abcam, Inc.) and b-catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology) at 4°C were used overnight. .. For immunofluorescence staining, cultured HK2 cells and serial cryo- stat sections of renal cortex were incubated with a primary rabbit antibody against EGFR (1:100 dilution; Cell Signaling Technology), EGFR-p (Tyr1068, 1:100 dilution; Cell Signal- ing Technology), and b-catenin-p (Ser33/ Ser37, 1:100 dilution; Cell Signaling Technology). .. For renal tubule segment identification, frozen kidney sampleswere incubatedwith pri- mary antibodies including FITC-conjugated anti-Lotus tetragonolobus lectin (1:500 dilu- tion; Vector Laboratories, Burlingame, CA) and FITC-conjugated anti-Dolichosbiflorus agglutinin (1:500 dilution; Vector Laboratories).31 The sections were counterstained with 49,6-diamidino-2-phenylindole (dilu- tion 1:500; Sigma-Aldrich) to identify cellular nuclei.

    Cell Culture:

    Article Title: Protein-Bound Uremic Toxins Induce Tissue Remodeling by Targeting the EGF Receptor
    Article Snippet: The Ventana BenchMark automated staining system and Ventana reagents were used (Ventana Medical Systems, Tucson, AZ), and primary antibodies against MMP2/MMP9 (1:100 dilution; Abcam, Inc.) and β -catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology) at 4°C were used overnight. .. For immunofluorescence staining, cultured HK2 cells and serial cryostat sections of renal cortex were incubated with a primary rabbit antibody against EGFR (1:100 dilution; Cell Signaling Technology), EGFR-p (Tyr1068, 1:100 dilution; Cell Signaling Technology), and β -catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology). .. For renal tubule segment identification, frozen kidney samples were incubated with primary antibodies including FITC-conjugated anti- Lotus tetragonolobus lectin (1:500 dilution; Vector Laboratories, Burlingame, CA) and FITC-conjugated anti- Dolichosbiflorus agglutinin (1:500 dilution; Vector Laboratories).

    Article Title: Protein-Bound Uremic Toxins Induce Tissue Remodeling by Targeting the EGF Receptor
    Article Snippet: For immunohistochemistry analysis, paraffin tissue sections were cut, mounted, deparaffi- nized, rehydrated, and stained with hematoxylin and eosin by using standard histologic techniques.TheVentanaBenchMark automated staining system and Ventana reagents were used (VentanaMedical Systems, Tucson, AZ), and primary antibodies against MMP2/ MMP9 (1:100 dilution; Abcam, Inc.) and b-catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology) at 4°C were used overnight. .. For immunofluorescence staining, cultured HK2 cells and serial cryo- stat sections of renal cortex were incubated with a primary rabbit antibody against EGFR (1:100 dilution; Cell Signaling Technology), EGFR-p (Tyr1068, 1:100 dilution; Cell Signal- ing Technology), and b-catenin-p (Ser33/ Ser37, 1:100 dilution; Cell Signaling Technology). .. For renal tubule segment identification, frozen kidney sampleswere incubatedwith pri- mary antibodies including FITC-conjugated anti-Lotus tetragonolobus lectin (1:500 dilu- tion; Vector Laboratories, Burlingame, CA) and FITC-conjugated anti-Dolichosbiflorus agglutinin (1:500 dilution; Vector Laboratories).31 The sections were counterstained with 49,6-diamidino-2-phenylindole (dilu- tion 1:500; Sigma-Aldrich) to identify cellular nuclei.

    Incubation:

    Article Title: Protein-Bound Uremic Toxins Induce Tissue Remodeling by Targeting the EGF Receptor
    Article Snippet: The Ventana BenchMark automated staining system and Ventana reagents were used (Ventana Medical Systems, Tucson, AZ), and primary antibodies against MMP2/MMP9 (1:100 dilution; Abcam, Inc.) and β -catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology) at 4°C were used overnight. .. For immunofluorescence staining, cultured HK2 cells and serial cryostat sections of renal cortex were incubated with a primary rabbit antibody against EGFR (1:100 dilution; Cell Signaling Technology), EGFR-p (Tyr1068, 1:100 dilution; Cell Signaling Technology), and β -catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology). .. For renal tubule segment identification, frozen kidney samples were incubated with primary antibodies including FITC-conjugated anti- Lotus tetragonolobus lectin (1:500 dilution; Vector Laboratories, Burlingame, CA) and FITC-conjugated anti- Dolichosbiflorus agglutinin (1:500 dilution; Vector Laboratories).

    Article Title: Protein-Bound Uremic Toxins Induce Tissue Remodeling by Targeting the EGF Receptor
    Article Snippet: For immunohistochemistry analysis, paraffin tissue sections were cut, mounted, deparaffi- nized, rehydrated, and stained with hematoxylin and eosin by using standard histologic techniques.TheVentanaBenchMark automated staining system and Ventana reagents were used (VentanaMedical Systems, Tucson, AZ), and primary antibodies against MMP2/ MMP9 (1:100 dilution; Abcam, Inc.) and b-catenin-p (Ser33/Ser37, 1:100 dilution; Cell Signaling Technology) at 4°C were used overnight. .. For immunofluorescence staining, cultured HK2 cells and serial cryo- stat sections of renal cortex were incubated with a primary rabbit antibody against EGFR (1:100 dilution; Cell Signaling Technology), EGFR-p (Tyr1068, 1:100 dilution; Cell Signal- ing Technology), and b-catenin-p (Ser33/ Ser37, 1:100 dilution; Cell Signaling Technology). .. For renal tubule segment identification, frozen kidney sampleswere incubatedwith pri- mary antibodies including FITC-conjugated anti-Lotus tetragonolobus lectin (1:500 dilu- tion; Vector Laboratories, Burlingame, CA) and FITC-conjugated anti-Dolichosbiflorus agglutinin (1:500 dilution; Vector Laboratories).31 The sections were counterstained with 49,6-diamidino-2-phenylindole (dilu- tion 1:500; Sigma-Aldrich) to identify cellular nuclei.



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    Image Search Results


    MFAP2 promotes epithelial–mesenchymal transition (EMT) through the EGFR-AKT-STAT3 signaling pathway in colorectal cancer (CRC) cells. (A, B) The top 20 enrichment signaling pathways regulated by MFAP2 knockdown. (C, D) VEGFR2 signaling pathway was enriched in the MFAP2 knockdown cells, shown by Gene Set Enrichment Analysis (GSEA) and essential genes in this enrichment. (E) MFAP2 knockdown affected the EGFR-AKT-STAT3 axis.

    Journal: Genes & Diseases

    Article Title: MFAP2 promotes metastasis and drug resistance by regulating epithelial-to-mesenchymal transition through EGFR signaling pathway in colorectal cancer cells

    doi: 10.1016/j.gendis.2025.101800

    Figure Lengend Snippet: MFAP2 promotes epithelial–mesenchymal transition (EMT) through the EGFR-AKT-STAT3 signaling pathway in colorectal cancer (CRC) cells. (A, B) The top 20 enrichment signaling pathways regulated by MFAP2 knockdown. (C, D) VEGFR2 signaling pathway was enriched in the MFAP2 knockdown cells, shown by Gene Set Enrichment Analysis (GSEA) and essential genes in this enrichment. (E) MFAP2 knockdown affected the EGFR-AKT-STAT3 axis.

    Article Snippet: Following blocking with 5% non-fat milk in PBS with 0.02% Tween 20 detergent (PBST) at room temperature for 2 h, the membranes were incubated with primary antibodies, including MFAP2 (Solarbio, China), GAPDH (BBI Co., Ltd., China), epidermal growth factor receptor (EGFR; Proteintech, China), protein kinase B (AKT) (Proteintech), signal transducer and activator of transcription 3 (STAT3) (Proteintech), and vascular endothelial growth factor A (VEGFA) (Proteintech), p-EGFR (Cell Signaling Technology, USA), p-STAT3 (Cell Signaling Technology), and p-AKT ser473 (Cell Signaling Technology) antibodies, at 4 °C overnight.

    Techniques: Protein-Protein interactions, Knockdown